- An ending where Eileen survived (Escape or Mother) must have been achieved in a previous game.
- The player must be playing a New Fear game.
After both conditions are met, Henry Townshend can find the nurse uniform inside of Eileen's apartment during his first visit to the Apartment World and Room 303. It will be lying on a chair beside the TV, and should be picked up before activating the scene with Eileen. In order to have Eileen wear the costume, however, the nurse costume must be in Henry's inventory through the end of the game in addition to, again, achieving an ending where Eileen survives. Essentially, Eileen can be seen wearing the costume during a third playthrough at the earliest.
- Anne Marie Cunningham dressed in this Nurse Uniform when she slept with Leonard B. Trent in Anne's Story.